After 5 days of osteoclastogenesis induction, osteoclast apoptosis in vitro was examined using the In-Situ Cell Death Detection Kit following the manufacturer’s instructions (#12156792910, Roche, Rotkreuz, Switzerland). Images were visualized under a fluorescence microscope (Leica Microsystems).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.