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Assessment of cognitive function by the Morris water maze test

MB Michael W. Beck
JD Jeffrey S. Derrick
RK Richard A. Kerr
SO Shin Bi Oh
WC Woo Jong Cho
SL Shin Jung C. Lee
YJ Yonghwan Ji
JH Jiyeon Han
ZT Zahra Aliakbar Tehrani
NS Nayoung Suh
SK Sujeong Kim
SL Scott D. Larsen
KK Kwang S. Kim
JL Joo-Yong Lee
BR Brandon T. Ruotolo
ML Mi Hee Lim
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Mice were subjected to the Morris water maze test to assess their performance of spatial learning and memory25,61. The water maze was a circular plastic pool (120 cm diameter) and filled with murky water (21.0±1.0 °C). A cylindrical escape platform (15 cm diameter) stood 0.5 cm under the water surface. Three hours after the 30th compound treatment, the mice were allowed the first training to swim and find the hidden escape platform in the water with three repeats per training. Thereafter, they daily tried the task on the next 4 consecutive days. Three hours after the final escape test, the platform was removed, and the mice exercised a probe trial for 60 s. The animal performance was traced with SMART Video Tracking System (Harvard Apparatus, Holliston, MA, USA).

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