The spinal cord tissue specimens were obtained 14 days after the SCI model was established, and frozen sections were prepared. Hematoxylin–eosin (H&E) staining was utilized to assess the histological manifestation. Immunofluorescence was applied to determine neuronal survival and scar formation after SCI. For the neuronal survival assessment, NeuN (ab177487, abcam) was used as the primary antibody, and Goat Anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary antibody, Alexa Fluor 488 (A-11008, Thermo Fisher) was labeled as the secondary antibody. For the scar formation assessment, GFAP (ab7260, abcam) was used as the primary antibody, and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077, abcam) was labeled as the secondary antibody. The Nissl body was evaluated using the Nissl staining kit (G1434, Solarbio) to assess the state of the neurons.
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