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Determination of Malondialdehyde and Superoxide Dismutase Contents
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Myocardial tissue homogenate was prepared by myocardial tissues and PBS (0.1 g/mL) through fully grounding in glass homogenizers. The myocardial tissue homogenate was centrifuged for 20 minutes at 3000 r/min at 4 °C, and the supernatants were collected for further detecting. The myocardial tissue malondialdehyde and superoxide dismutase contents were determined using malondialdehyde and superoxide dismutase assay kits (Cayman Chemical), respectively, according to the manufacturer's instruction.
Copyright and License information: ©2023 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
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