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The correlation between AD and MS in immune cell infiltration compared with normal tissues
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A bioinformatics algorithm called "CIBERSORT" was used to evaluate immune cell infiltrations. The leukocyte gene signature matrix LM22 with 1,000 permutations was used to calculate the putative abundance of immune cells24. Data with "CIBERSORT" values of p < 0.05 were filtered and retained for subsequent analysis. We conducted Spearman's rank correlation analysis of the disease hub genes and immune cells to further explore the immune mechanism during the development of AD with MS. The results were visualized with the "corrplot" package.
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