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Using the Illumina HiSeq 2000 Sequencer, WES was conducted on DNA extracted from the APA along with paired germline DNA extracted from venous blood (samples processed commercially by GATC Biotech). WES samples were prepared as an Illumina sequencing library, and sequencing libraries were enriched using the Agilent SureSelectXT Human All Exon V6 Kit. The captured libraries were sequenced and downstream analysis conducted as described below.
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