We photoconverted fish expressing pnp4a:nucEos stably or mosaically using a Zeiss LSM 800 scanning laser confocal with a 405 nm laser and ZEN blue software. When we photoconverted entire flanks (Supplementary Fig. 3), we placed fish in a box lined with aluminum foil and exposed them to full intensity output from a Zeiss HXP 120 V compact light source.
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