Photoconversion

DG Dvir Gur
EB Emily J. Bain
KJ Kory R. Johnson
AA Andy J. Aman
HP H. Amalia Pasolli
JF Jessica D. Flynn
MA Michael C. Allen
DD Dimitri D. Deheyn
JL Jennifer C. Lee
JL Jennifer Lippincott-Schwartz
DP David M. Parichy
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We photoconverted fish expressing pnp4a:nucEos stably or mosaically using a Zeiss LSM 800 scanning laser confocal with a 405 nm laser and ZEN blue software. When we photoconverted entire flanks (Supplementary Fig. 3), we placed fish in a box lined with aluminum foil and exposed them to full intensity output from a Zeiss HXP 120 V compact light source.

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