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Cell lysates were mixed with the indicated antibody and subsequently 20‐μl protein A/G‐agarose beads (Santa Cruz Biotechnology) and incubated on a rotator for 4 h at 4°C. The beads were washed twice with PBS and then twice with lysis buffer supplemented with complete mini protease inhibitor cocktail. Bound proteins were boiled in sample preparation buffer for 5 min and then used for western blotting analysis.
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