MSBB

Samples were collected at NBTR following similar parameters to MSBB–Mount Sinai NIH Neurobiobank cohort ⁶¹. Collected autopsies were selected to represent a full spectrum of cognitive and neuropathological disease severity in the absence of discernible non-AD neuropathology. All neuropsychological, diagnostic and autopsy protocols were approved by the Mount Sinai and JJ Peters VA Medical Center Institutional Review Boards, with neuropathological assessments, cognitive, and medical and neurological status determinations performed according to previously published procedures. Postmortem brain tissue was placed in an ice-cooled insulated box and transported from the site of recovery to the Neurobiobank laboratory. A section of the frontal pole (Brodmann area 10) was dissected in the Neurobiobank laboratory, and rinsed in ice-cold sterile saline, placed in pre-cooled (4°C) MACS tissue storage solution (Miltenyi Biotec, cat.# 130-100-008), and immediately refrigerated (4°C).