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Four buffers were used for sample preparation and imaging: Buffer A (10 mM Tris-HCl pH 7.5, 100 mM NaCl); Buffer B (5 mM Tris-HCl pH 8.0, 10 mM MgCl2, 1 mM EDTA); Buffer C (1× PBS, 500 mM NaCl); 10x folding buffer (100 mM Tris,10 mM EDTA pH 8.0, 125 mM MgCl2). Buffers were checked for pH. Imaging buffers were supplemented with oxygen scavenging & triplet state quenching system 1× PCA, 1× PCD, 1× Trolox prior to imaging.
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