Triacsin C Treatment

Triacsin C (TriC) was purchased from Cayman Chemical (Ann Arbor, Michigan, USA). MM.1S^gfp+/luc+, MM.1R^gfp+/luc+, OPM-2, RPMI-8226, and U266B1 cells were seeded into either tissue culture-treated white bottom 96 well plate (4.33x10⁴ cells/well), tissue culture treated 24 well plates (1x10⁵ cells/well), tissue culture-treated 6-well dishes (4.81x10⁵ cells/well), or tissue culture-treated T-25 flasks (2x10⁶ cells/flask; Avantor/VWR, Cat. No. 10861–568) under the growth conditions described above. MM cells were treated with TriC or dimethyl sulfoxide (DMSO, vehicle). Samples were collected at 24-hour intervals and subjected to functional analyses below.