Neurite outgrowth assay

The quantification of neurite was performed as described before [⁸]. Shortly, 1 × 10⁴ SK-N-BE(2) and NB16 cells were plated in a 12 well-plate per well. After overnight incubation, cells were treated with various conditions as indicated for 72h. Then, images were taken by a Leica Florescent Microscope DMi8 in phase contrast mode (20× magnification). The lengths of the neurites were traced and quantified using the ImageJ plugin NeuronJ[¹⁵]. For each sample, total neurite length was measured and normalized to the number of cell bodies. Data are presented as mean ± SD of three biological repeats.