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Cell viability assays were performed by seeding 5,000 cells/well in a 96-well opaque walled plate. Replicates were then treated with the BRAF inhibitor, dabrafenib (Sellekchem S2807), MEK inhibitor, trametinib (Selleckchem S2673), or combined treatment. The CellTiter-Glo Luminescent Viability Assay (Promega G7571) was used to measure cell viability at 24, 48, and 72 hours following the manufacturer’s protocol. Luminescence was measured using the Tecan M1000 Pro Microplate Reader.
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