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To determine the concentration of SCFAs in the stool samples, 50–100mg of fecal matter were aliquoted from every stool sample and sent to the University of Pittsburgh Health Sciences Metabolomics and Lipidomics Core in the Department of Pharmacology & Chemical Biology. The fecal acetate, butyrate, propionate and valerate levels were measured by stable isotope dilution liquid chromatography mass spectrometry43.
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