Construction of Tmem30a KD mouse podocytes

Tmem30a was knocked down in immortalized mouse podocytes using lentivirus-mediated RNA interference, and phenotypic analysis and functional verification were performed at the cellular level. Cells were transfected for 72 h with the interfering virus, after which Tmem30a knockdown was verified using RT-PCR and western blotting. Differentiated podocytes (control group), transfected negative control lentivirus mouse podocytes (NC group), and Tmem30a KD mouse podocytes (Tmem30a KD group) were treated with 0.4 μg/mL of ADR for 24 h. They were then treated with 100 μM of the NLRP3 inhibitor MCC950 (Selleck) for another 48 h or 300 nM of the GSDMD inhibitor DSF (Selleck) for 1 h prior to model.