Immunohistochemistry

Differentially treated EAE mice were anesthetized with chloral hydrate (3 µg/g via intraperitoneal injection) and the spinal cords were removed and fixed in 4% paraformaldehyde and paraffin embedded. Sections were stained to detect the myelin, oligodendrocytes, and inflammatory cells. The following primary antibodies were utilized in immunolabeling: Rabbit anti-mouse MBP antibody (ab40390, Abcam), Rabbit anti-mouse Oligodendrocyte Specific Protein antibody (ab53041, Abcam), and rabbit anti-mouse CD3 antibody (ab5690, Abcam). The secondary antibody is Cy3-AffiniPure Goat Anti-Rabbit IgG (JAC-111165045, ELITechGroup). Sections were examined under an Olympus BX51 microscope (Olympus).