Tumor xenograft experiment

A549 cells were prepared and injected subcutaneously into nude mice (1×10⁷ A549 cells in 200 μl PBS per mouse). The experimental group mice received 25 μg/ml of Taraxasterol in drinking water. Tumor growth was recorded every two days. Until the tumor reached a diameter of over 1 cm, approximately 30-40 days post cells injection. After the tumor was drawn, tumors were weighed, photographed, and divided into three parts: one for total RNA extraction, one for protein extraction (both preserved in liquid nitrogen), and the third for HE sectioning and immunohistochemistry, which was fixed in 4% PFA for half an hour.