Flow cytometry

The single-cell suspension was incubated with PBS containing the antibody cocktail for 20 minutes at 4°C in the dark. Cells were washed with PBS twice and fixed using Cytofix/Cytoperm buffer (BD). Cells were washed 2 times with Perm/wash buffer (BD). Cells were ultimately collected in FACS buffer (PBS w/o Ca2⁺ Mg2⁺ with 5mM EDTA) before acquisition. Samples were acquired using LSR Fortessa (BD Bioscience), and data was analyzed using FlowJo v10 (FlowJo). Analyses were all performed after the exclusion of dead cells and doublets. The list of used antibodies is displayed in Table S4.