Particle Size Measurements by Dynamic Light Scattering

The droplet size of the resulting emulsions was characterized by dynamic light scattering (DLS) using a Zetasizer Nano-ZS instrument (Malvern Instruments, Southboro, MA). For measurement, samples were diluted in DCM-saturated water to a final solids concentration of 0.3 mg mL^–1. Emulsions were characterized by DLS immediately after preparation, as well at “intermediate” (t = 3–5 h) and “long” (t > 18 h) time points to assess the size stability of the emulsions. Data is reported as intensity weighted particle size distributions with Z-average hydrodynamic diameter. The broadness of the size distribution is characterized by the polydispersity index (PDI); PDI < 0.1 indicates a monodisperse sample, while PDI > 0.7 generally indicates a distribution too broad for accurate DLS measurement. Cumulants analysis of DLS data was performed by the Zetasizer software package in accordance with ISO 13320.