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2.5. Wound Healing Assay

RD Ravi Doddapaneni
JT Jason D. Tucker
PL Pei J. Lu
QL Qi L. Lu
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In vitro migration (scratch) assay was carried out in MDA-MB-231 cells. The cells were seeded in 12-well culture plates in complete media and incubated overnight. After reaching confluence, a uniform scratch was made in the center of the well using a micropipette tip, and a baseline image was taken of the entire scratch width. Cells were then treated with half of the EC50 concentrations determined for JQ1. Cells were incubated for 48 h in a MuviCyte (PerkinElmer, Waltham, MA, USA) Live-Cell imaging system attached to an incubator at 37 °C in 5% CO2. Microphotographs were captured at 2 h intervals via the MuviCyte Live-Cell imaging software version 2.0.26 (PerkinElmer) and analyzed.

Copyright and License information:  ©2023 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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