2.2. Wound Healing Assay

A total of 1.5 × 10⁵ ovarian cancer cells per well were plated in 12-well plates. After 16 h, scratches were generated with a 100 µL pipette tip. Ovarian cancer cells were co-cultured with HMC-1 (cell-to-cell ratio 1:1 or 1:5) directly or by using 0.4 µm pore-size transwells (Corning, NY, USA). Ovarian cancer cells alone served as controls. Images of scratch areas were taken after 0 h, 24 h, and 48 h with the light microscope and Axio Vision software version 4 (Zeiss, Jena, Germany).