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Preparation of PMCA substrate
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Perfused whole brains of TgVole 1 × mouse model (see details on the model in the next section), transgenic mice expressing onefold bank vole I109 PrP under the control of the murine PrP promoter in a murine Prnp0/0 background [30], were homogenized at 10% (w/v) in CB with protease inhibitor cocktail (Roche) in a glass potter pestle (Fisher scientific), aliquoted and stored at − 80 °C until required.
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