Whole transcriptome library preparation and sequencing

Total RNAs from 3 ECRSwNP, 3 non-ECRSwNP and 3 control subjects were isolated and quality controlled. The preparation of whole transcriptome libraries and deep sequencing were performed by Novogene Bioinformatics Technology Cooperation (Beijing, China). Ribosomal RNA (rRNA) was removed and strand-specific sequencing libraries were generated following manufacture’s recommendations. RNA-Seq was performed on an Illumina Hiseq 2000 platform and 100 bp paired-end reads were generated according to Illumina’s protocol. Detailed procedures are in the Supplementary Information. The NCBI Gene Expression Omnibus accession number for the RNA-Seq data reported in this paper is {"type":"entrez-geo","attrs":{"text":"GSE72713","term_id":"72713"}}GSE72713.