Histology.

Mice were anaesthetized (sodium pentobarbital; 100 mg per kg and 20 mg per kg body weight) and perfused transcardially with 4% paraformaldehyde in phosphate buffer, pH 7.4. For anatomical tracing experiments, coronal sections (50–75 µm) were cut using a vibratome (VT1000; Leica). For chemogenetic inactivation experiments, coronal sections (150 µm) were cut, tissue cleared and embedded in hydrogel using PACT-CLARITY, and stained for Fos (B4-Alexa647 hairpin amplifiers) using HCR-FISH as previously described²⁷. Images were acquired using a epifluorescent microscope (Eclipse NiE, Nikon) or spinning disk confocal microscope (Ti2-E Yokogawa Spinning Disk, Nikon).