Other statistical analyses

One-way analysis of variance and Spearman’s correlation tests were performed using Sidak’s adjustment for multiple comparisons⁶⁸. Hypergeometric tests were performed using an online tool (https://systems.crump.ucla.edu/hypergeometric/). Promoter rankings were given based on |log₂ fold change| for promoters with an FDR < .05 in the all-replicate comparison and a minimum barcode number of 20. Heatmaps were generated using Morpheus⁶⁹. Hierarchical clustering was performed via Morpheus using Euclidean distance with complete linkage. Dose response curves were generated using GraphPad Prism 9.5.0 using three or four parameter models.