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H&E staining

MS Merrick Strotton
TH Tsuyoshi Hosogane
MM Marco di Michiel
HM Holger Moch
ZV Zsuzsanna Varga
BB Bernd Bodenmiller
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Sample sections on Mylar film after XRF imaging were cut and taped flat onto a glass slide for H&E staining. The glass slide was stained with hematoxylin for 3 min, then washed in deionized water for 1 min. Bluing was performed in Scott’s tap water for 1 min, followed by washing in deionized water for 1 min and in 95% ethanol for 1 min. The slide was then stained with eosin for 1 min, washed with 100% ethanol for 2 min, then with Ultraclear (J.T. Baker, catalog no. 3905.5000PE) for 4 min. The slide was mounted with Eukitt (Sigma, catalog no. 03989), and the bright-field image obtained with a slidescanner (Zeiss, Axio Scan.Z1). The horizontal dimension of H&E images was scaled by 0.9 to match XRF images (which were collected at a 28° angle).

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