PhenoCycler 2 (PCF-Human Intestine)27:

Data from 64 sections of the human intestine were collected from 8 donors (B004, B005, B006, B008, B009, B010, B011, and B012). In this study, the authors used spatial proteomics to examine the structure of the large and small intestines in humans. The raw image data were segmented using either the CODEX Segmenter or the CellVisionSegmenter (available at https://github.com/nolanlab/CellVisionSegmenter). Employing a 57-marker CODEX panel, a total of 2,603,217 cells were profiled. These cells were initially grouped using Leiden clustering and subsequently annotated under the supervision of the authors⁴⁰. To ensure accuracy, the cell type labels were further consolidated by the authors by inspecting back-annotated cell types on the original images. The list of signatures cell types was provided in the original paper and expert domain knowledge. PCF-HI can be download at: https://datadryad.org/stash/dataset/doi:10.5061/dryad.pk0p2ngrf.