2.5. Inoculum preparation

A glucose rich solid medium was used (peptone 10 g, glucose 20 g, agar 20 g, KH₂PO₄ 0.5 g, H₂O 1 L, adjusted to pH 4.0 with HCl before autoclaving) [23]. This medium was poured into 300 plastic Petri plates, agar allowed to harden, and then plates were inoculated by subculturing from the growing margins of three-day-old colonies growing on PDA and then incubated at room temperature (23 ± 2 °C).