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Cell lines and cell cuture

XZ Xin Zhang
WG Wenjie Gu
AL Aiqin Lin
RD Renjie Duan
LL Likai Lian
YH Yuanyuan Huang
TL Tiechen Li
QS Qing Sun
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The human SKOV3 and A2780 ovarian cancer cell lines and the IOSE80 normal ovarian epithelial cell line were purchased from Wuhan Procell Life Science&Technology (Wuhan, China). The OVCAR-3 cell line was obtained from the Cell Resource Center of Shanghai Institute of Life Sciences Chinese Academy of Sciences (Shanghai, China). The SKOV3 cell line was cultured in McCoy’s 5 A medium (Gibco, Carlsbad, CA, USA), the A2780 cell line was cultured in 1640 medium (Gibco, Carlsbad, CA, USA), and the IOSE80 cell line was cultured in DMEM (Gibco, Carlsbad, CA, USA). The three cell lines were cultured in medium supplemented with 10% fetal bovine serum (Gibco, Carlsbad, CA, USA) and 1% penicillin and streptomycin. The OVCAR-3 cell line was cultured in 1640 medium (Gibco, Carlsbad, CA, USA) supplemented with 20% fetal bovine serum and 0.01 mg/ml insulin. The cells were cultured in an incubator at 37 °C in 5% CO2.

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