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For the CCK8 proliferation assay, BMSCs (3000 cells per well) were seeded in 96‐well plates and incubated at 37°C with 5% CO2. The plate was subsequently processed with Cell Counting Kit 8 (CCK8) solution (10 μL; Dojindo, Japan) for specified durations (0, 12, 24, 36 and 48 h) at 37°C. Optical density was measured at 450 nm to calculate the percentage of cell viability on a microplate reader (Spectramax, USA).
Copyright and License information: ©2023 The Authors. published by Beijing Institute for Stem Cell and Regenerative Medicine and John Wiley & Sons Ltd.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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