5.9. Determination of Protein Expression by Immunofluorescence

The cells were cultured for 24 h before being incubated with the primary antibody CytC (1:100) at 4 °C overnight, followed by anti-rabbit secondary antibody iFluor™ 488 (1:800) at 37 °C for 1 h. The fluorescent signals were examined via a digital slide scanner (Pannoramic MIDI, 3DHISTECH, Budapest, Hungary). The details were reported by our laboratory’s previous study [5].