Biotinylated human MR1/5-OP-RU and MR1/6-FP monomers were provided by the NIH Tetramer Core Facility. Tetramers were generated using streptavidin-PE (high concentration) or streptavidin-BV421 (both BioLegend) following the NIH Tetramer Core Facility protocol. Tetramer staining was performed for 40 min at 21 °C in FACS buffer (PBS, 0.5% BSA (Sigma-Aldrich), 1 mM EDTA (Sigma-Aldrich)).
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