Tetramer staining (Exp 1 and 2)

LG Lucy C. Garner
AA Ali Amini
MF Michael E. B. FitzPatrick
ML Martin J. Lett
GH Gabriel F. Hess
MS Magdalena Filipowicz Sinnreich
NP Nicholas M. Provine
PK Paul Klenerman
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Biotinylated human MR1/5-OP-RU and MR1/6-FP monomers were provided by the NIH Tetramer Core Facility. Tetramers were generated using streptavidin-PE (high concentration) or streptavidin-BV421 (both BioLegend) following the NIH Tetramer Core Facility protocol. Tetramer staining was performed for 40 min at 21 °C in FACS buffer (PBS, 0.5% BSA (Sigma-Aldrich), 1 mM EDTA (Sigma-Aldrich)).

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