2.1. Plasmids, Reagents and Compounds

pBABE-puro-NLS-LSSmKate2 was a gift from Vladislav Verkhusha (Addgene plasmid #34586). Gα_i1 (#GNAI100000), Gα_i2 (#GNAI200000), Gα_i3 (#GNAI300000), Gα_OA (#GNA0OA0000), Gα_OB (#GNA0OB0000), Gα_Ss (#GNA0SS0000), Gα_SL (#GNA0SL0000), Gα₁₂ (#GNA1200000), Gα₁₃ (#GNA1300001), Gα_q (#GNA0Q00000), Gα₁₅ (#GNA1500000) Gγ1 (#GNG0100000), Gγ2 (#GNG0200000), Gγ9 (#GNG0900000), Gβ1 (#GNB0100000), β2-adrenergic receptor (#AR0B200000), Thromboxane A2 receptor (#TXA2R00000) and Histamine 3 receptor (#HRH0300000) were purchased from cDNA Resource Centre and are in the pcDNA3.1(+) backbone. The pNLF1-N vector (#N1351) and pGlosensor-22F cAMP plasmid (#E2301) were purchased from Promega. Pertussis toxin (PTX; #3097) was purchased from Tocris, Bristol, UK, Cholera Toxin (CTX; #C8052) from Merck, Rahway, NJ, USA and YM-254890 (#257-00631) from FUIJIFILM Wako Chemicals, Osaka, Japan. Histamine dihydrochloride (Histamine; #3545) was purchased from Bio-Techne, Minneapolis, MN, USA; Isoprenaline hydrochloride (Isoprenaline; #I5627), 9,11-Dideoxy-11α,9α-epoxymethanoprostaglandin F2α (U46619; #D8174), 3-isobutyl-1-methylxanthine (IBMX; #I7018), Forskolin (FSK; #F6886) and Poly-D-Lysine (PDL; #2780) from MERCK. Nano-Glo^® Vivazine™ substrate (#N2581) and NanoBRET™ Nano-Glo^® Substrate (#N1571) were purchased from Promega, Madison, WI, USA.