Clustal omega alignments and EMBOSS NEEDLE pair-wise alignments

E. coli ({"type":"entrez-protein","attrs":{"text":"P27296","term_id":"544162","term_text":"P27296"}}P27296) and S. aureus ({"type":"entrez-protein","attrs":{"text":"Q2FGY5","term_id":"123485907","term_text":"Q2FGY5"}}Q2FGY5) DinG sequences were obtained through the Uniprot database server and aligned to P. aeruginosa 83 CasDinG using the Clustal Omega multiple sequence alignment tool (19). Pairwise sequence alignments were performed between Pa83 CasDinG and E. coli or S. aureus sequences using an EMBOSS Needle alignment (20). Structural alignments between domains were either performed with the Secondary Structure Matching tool in Coot (21,22), the Dali server (23), or the align command in the PyMOL Molecular Graphics System, Version 2.0 Schrödinger, LLC.