Knockdown by siRNA

TH Takeshi Harada
HY Hideki Yamamoto
SK Shosei Kishida
MK Michiko Kishida
CA Chihiro Awada
TT Toshifumi Takao
AK Akira Kikuchi
request Request a Protocol
ask Ask a question
Favorite

CHO and Caco‐2 cells were transfected with a mixture of siRNAs (20 nM each) against genes of interest using RNAiMAX (Invitrogen, Grand Island, NY, USA) and the cells were used for experiments 48 h post‐transfection. Target sequences are listed in Table S2.

Supplementary materials and methods are described in Data S1.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A