Following hospital discharge, YF patients were initially advised to return at 30, 45, and 60 days post–symptom onset (dps) for ambulatory follow-up, but in some cases the follow-up period was extended. During follow-up, clinical examination, complete blood cell count, and liver and renal function tests were performed. Serum samples were collected during acute disease hospitalization and ambulatory follow-up and kept at −70°C. The samples were further used for qualitative and quantitative RT-qPCR, for the investigation of YFV RNA, as described below.
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