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4.8. Vaginal Local Characteristics and CFU Count
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Fungal loading in mouse vaginal lavage fluid was determined using the plate method. Specifically, 30 μL of mouse vaginal lavage fluid was diluted in PBS, coated on YPD agar medium, and incubated in a 37 °C incubator for 24 h to quantify the fungal load (CFU/mL) [39].
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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