Statistical Analysis

Values are presented as mean ± SEM from at least 3 independent experiments. Statistical analyses were performed by using GraphPad Prism Software (San Diego, CA, USA). Statistical significance of the data was analyzed with Kruskal-Wallis non-parametric test with Dunn’s post-test for time curve of cortical exocytosis, Student’s t-test for cortical exocytosis measurement and the internalized fluorescent dots in pulse-chase assays and one-way ANOVA for pulse-chase experiments in fertilized eggs. In pulse-chase assays with inhibitors, relative internalized fluorescent labeling was considered statistically different to control whenever the 95% confidence interval did not include 1. In all cases, differences were considered significant at p<0.05.