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Chromatin immunoprecipitation was performed as previously described (15). Chromatin was prepared and immunoprecipitated with the following modifications: 50 fly heads, representing equal numbers of males and females, were homogenized before formaldehyde cross-linking. Chromatin was sheared to 500 bp by 15- × 30-s bursts of sonication at 100% duty level 5 power with a Branson sonicator. IgG was used as a negative control, and data were normalized to input.
Copyright and License information: ©2020
Published under the PNAS license.
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