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After SBI, 150 µL of 1% Evans Blue in PBS was injected intraorbitally. Two hours later, mice were perfused with PBS followed by PFA. Brains were removed, imaged, and dried at 60 °C overnight. The Evans blue dye was then extracted using 1 mL formamide at 55 °C for 16 h and quantified using a spectrophotometer at 630 nm [15]. Evans blue optical density was then divided by dry weight to calculate BBB disruption.
Copyright and License information: The American Society for Experimental NeuroTherapeutics, Inc. ©2022
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