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ZnP@DHA, ZnP@Pyro-Fe, or ZnP@DHA/Pyro-Fe with or without 5 mM cysteine was dispersed in 0.5% Triton X-100 that contains 10 μM H2-DCFDA (Thermo Fisher Scientific, USA). The ROS generation in the presence or absence of O2 was investigated by determining the change of DCF fluorescence over time.
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