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Measurements of IL-33, IL-17, IFN-γ, IL-5, IL-6, and IL-13 concentrations in BAL fluid after exposure to Alternaria or IL-33 were performed with Quantikine ELISA kits (R&D Systems, Minneapolis, Minn), following the manufacturer’s instructions. Protein concentrations in the BAL fluid were quantitated with a BCA Protein Assay Kit (Bio-Rad, Hercules, Calif).
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