Human NSCLC cell lines A549 (EGFR high expression) and H1299 (EGFR low expression) were acquired from American Type Culture Collection (Manassas, VA, USA). All cell lines were maintained in RPMI 1640 supplemented with 12% fetal bovine serum (FBS; Invitrogen, CA, USA), 1% sodium pyruvate, and 1% antibiotic/antimycotic solution and maintained at 37 °C with 5% CO2 incubator.
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