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Measurement of the intracellular level of GSH and the GSH/GSSG ratio.

XX Xi-Qiu Xu
TX Tongran Xu
WJ Wenting Ji
CW Chong Wang
YR Yujie Ren
XX Xiaobei Xiong
XZ Xi Zhou
SL Shu-Hai Lin
YX Yi Xu
YQ Yang Qiu
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The intracellular level of GSH was determined using a GSSG/GSH quantification kit (Dojindo Laboratory) according to the manufacturer’s instructions. Briefly, cells were lysed in 10 mM HCl and subjected to 2 freeze-thaw cycles, followed by treatment with 5% sodium sulfosalicylate. For GSSG measurement, a masking solution was first added to the supernatant samples. After that, the samples were transferred to a 96-well plate, and buffer solution was added to each well containing the supernatant samples, allowing incubation for 1 h at 37°C. The substrate solution and coenzyme/enzyme working solution were then added to each well, and the samples were incubated for another 10 min at 37°C. Thus, total GSH and GSSG levels and their standards were measured using a plate reader at a wavelength of 412 nm.

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This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

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