Identification of KoNAC genes in K obovata

The complete genome data of K. obovata were retrieved from the Genome Sequence Archive database (https://bigd.big.ac.cn/gsa/browse/CRA002395). The NAC gene sequences of A. thaliana and rice were searched and downloaded from the Arabidopsis Information Resource (TAIR) website (http://www.Arabidopsis.org) and the Rice Genome Annotation Project (http://rice.plantbiology.msu.edu/), respectively. A hidden Markov model (HMM) profile of the Pfam NAC domain (PF01849) and NAM domain (PF02365) downloaded from the Pfam database (http://pfam.sanger.ac.uk/) was employed to identify KoNAC genes by using HMMER 3.2.1 with the default parameters, and the cutoff value was set to 0.01. The putative KoNACs were further validated by screening for NAC and NAM domains in the Pfam database (http://pfam.xfam.org/) and were finally confirmed as the KoNAC proteins after removing redundant sequences with CD-HIT program (http://cd-hit.org/) and performing BLASTP searches. The physicochemical properties of the KoNAC proteins, including their molecular weight (MW) and isoelectric point (pI), were analyzed with the online software ExPASy (https://www.expasy.org). The subcellular localization of the KoNAC proteins was determined with the PSORT program (https://psort.hgc.jp/).