Cell aggregation assay

QD Qu Deng
RN Ramakrishnan Natesan
FC Florencia Cidre-Aranaz
SA Shehbeel Arif
YL Ying Liu
RR Reyaz ur Rasool
PW Pei Wang
EM Erick Mitchell-Velasquez
CD Chandan Kanta Das
EV Endrit Vinca
ZC Zvi Cramer
PG Patrick J. Grohar
MC Margaret Chou
CK Chandan Kumar-Sinha
KW Kristy Weber
TE T.S. Karin Eisinger-Mathason
NG Nicolas Grillet
TG Thomas Grünewald
IA Irfan A. Asangani
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24-well plates were coated with 100 μL of 3% of poly-HEMA and dried in cell culturing hood overnight. 10,000 cells in single cell suspension were seeded in the wells, pictures were taken at time 0 and 16hr after seeding. Aggregation sizes were measured with ImageJ, student t-test was used for statistical analysis.

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