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Cleavage site analysis

EH Eisuke Hayakawa
CG Christine Guzman
OH Osamu Horiguchi
CK Chihiro Kawano
AS Akira Shiraishi
KM Kurato Mohri
ML Mei-Fang Lin
RN Ryotaro Nakamura
RN Ryo Nakamura
EK Erina Kawai
SK Shinya Komoto
KJ Kei Jokura
KS Kogiku Shiba
SS Shuji Shigenobu
HS Honoo Satake
KI Kazuo Inaba
HW Hiroshi Watanabe
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To assess actual cleavage sites in neuropeptide precursors in an unbiased way, we focused on neuropeptide structures that had been identified by mass spectrometry-based systematic peptide identification. AA sequences of neuropeptides were acquired from Homo sapiens, Drosophila melanogaster (Arthropoda) and Lineus longissimus (Nemertea). N-terminal and C-terminal cleavage sites (2 AA) and flanking regions (6 AA) of the collected neuropeptides in their precursors were extracted and subjected to composition analysis or sequence logo analysis with WebLogo57. The sources of peptide information for each animal species and the details of the cleavage site analysis are described in the Supplementary Information.

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