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Generation of SAMHD1 knock-down cells by RNA interference (RNAi)
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For SAMHD1 knock-down in MCF7 cells, siRNAs targeting SAMHD1 gene (siSAMHD1) and non-targeting control (siNT) (siGENOME SMARTpool; Dharmacon, Cultek) were transfected following standard procedures. In brief, 100 nM of the corresponding siRNA were mixed with Lipofectamine 3000 reagent (cat num. L3000001, ThermoFisher) and added to 200,000 MCF7 cells seeded in 24-well plates in Optimem medium without FBS. After 24h, complete DMEM was added and SAMHD1 depletion evaluated by RNA and protein expression.
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