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AllPrep DNA/RNA/miRNA universal kit
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The AllPrep DNA/RNA/miRNA Universal Kit (QIAGEN) is a widely used spin column-based method that allows simultaneous purification of DNA and RNA from cells or tissue samples. DNA and RNA extraction was performed according to manufacturer’s instructions. Elution volumes for the DNA fraction were two volumes of 100 µl if the eluate was to be used for assessment of yield, and 50 µl if the eluate was to be used for qPCR. Elution volumes for the RNA fraction were 50 µl.
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