Scanning electron microscopy

Primary hippocampal neuron number and morphology were assessed by SEM, as previously described (Han et al., 2019). After incubation on PCL NF membranes for 72 hours, cells were washed twice with PBS, followed by immersion in PBS containing 1% glutaraldehyde for 4 hours. Samples were then dehydrated using a 20–100% ethanol series, washed, and air-dried at 25°C for 1 hour. The samples were spattered with gold in a vacuum and observed by Scanning electron microscopy (SEM). Three biological repeats were performed for each group, with three random fields of view for each slice photographed.